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Product Info Summary
| SKU: | CI1058 |
|---|---|
| Size: | 50μg/72μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ChIP, ELISA, WB |
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Product info
Product Name
Anti-MBD1 Antibody
SKU/Catalog Number
CI1058
Size
50μg/72μl
Form
Liquid
Description
Boster Bio Anti-MBD1 Antibody (Catalog# CI1058). Tested in ChIP, ELISA, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C. For long-term storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Cite This Product
Anti-MBD1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # CI1058)
Host
Rabbit
Contents
Affinity purified polyclonal antibody in PBS containing 0.05% azide and 0.05% ProClin 300.
Clonality
Polyclonal
Immunogen
This antibody is raised in rabbit against human MBD1 (Methyl-CpG-binding domain protein 1), using a KLH-conjugated synthetic peptide containing a sequence from the N-terminal part of the protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
CI1058 is reactive to MBD1 in Human, Mouse
Applications
CI1058 is guaranteed for ChIP, ELISA, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
66.607kDa
Background of MBD1
MBD1 is a transcriptional repressor that specifically binds to methylated CpG dinucleotides in promoter sequences. MBD1 acts by recruiting a variety of histone deacetylases (HDAC’s) and chromatin remodelling factors. MBD1-dependent transcriptional repression is mediated by ATF7IP through the recruitment of factors such as the histone methyltransferase SETDB1. MBD1 probably forms a complex with SETDB1 and ATF7IP which couples DNA methylation to H3K9 trimethylation and represses transcription.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
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ChIP assays were performed using human osteosarcoma (U2OS) cells, Anti-MBD1 polyclonal antibody (Catalog # CI1058) and optimized PCR primer sets. Sheared chromatin from 1x10e6 cells and 1.5 μg of antibody were used per ChIP experiment. Beads only were used as a negative IP control. Quantitative PCR was performed with primers for the promoter of the MLH1 gene, used as a positive control, and for the promoter of the CDC6 gene, used as a negative control.
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To determine the titer of the antibody, an ELISA was performed using a serial dilution of Anti-MBD1 polyclonal antibody (Catalog # CI1058), crude serum and Flow Through. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1:20,000.
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Western blot analysis of MBD1 expression in histone extracts from HeLa cells (40 μg). MBD1 was detected using Anti-MBD1 polyclonal antibody (Catalog # CI1058) at 1/1000 dilution.
Protein Target Info & Infographic
Gene/Protein Information For MBD1 (Source: Uniprot.org, NCBI)
Gene Name
MBD1
Full Name
Methyl-CpG-binding domain protein 1
Weight
66.607kDa
Alternative Names
CXXC-type zinc finger protein 3; methyl-CpG binding domain protein 1 isoform PCM1; methyl-CpG binding domain protein 1; methyl-CpG-binding domain protein 1; Methyl-CpG-binding protein MBD1; PCM1CXXC3RFT; Protein containing methyl-CpG-binding domain 1; the regulator of fibroblast growth factor 2 (FGF-2) transcription MBD1 CXXC3, PCM1, RFT methyl-CpG binding domain protein 1 methyl-CpG-binding domain protein 1|CXXC-type zinc finger protein 3|protein containing methyl-CpG-binding domain 1|the regulator of fibroblast growth factor 2 (FGF-2) transcription
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MBD1, check out the MBD1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MBD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MBD1 Antibody (CI1058)
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No publications found for CI1058
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Customer Reviews
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